DNA
Part:BBa_K2460011
Designed by: Haiyun Liu Group: iGEM17_Fudan_China (2017-10-24)
φC31 attP
This part is improved from BBa_K1039000.
This is a recombination site attP for phiC31 integrase (BBa_K1039012). When used with phiC31 attB (BBa_K2460010) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiC31 integrase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |